Fluorescence Lifetime Imaging
XiaoTao Hao, Lachlan McKimmie, Allison Heskes
In addition to being able to measure the fluorescence lifetimes of bulk solutions or films, using a scanning microscope we are also able to generate lifetime images of fluorescent samples. These images give additional information over intensity based images as a fluorescence decay profile is collected for every pixel. Examining the changes in fluorescence lifetimes across the image aids in determining the local environments of the fluorophores. The image below is of a wool fibre; here the different colours represent different fluorescent lifetimes.
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