Major Research Techniques

Spectroscopy

  • Multi-Colour and White Light Pump-probe transient absorption (femtoseconds to seconds)
  • Multi-Colour Spectrally Resolved Three-Pulse Photon Echo Peak Shift
  • Femtosecond Kerr-gated fluorescence
  • Femtosecond fluorescence upconversion
  • Stimulated emission decay measurements
  • Picosecond fluorescence measurements by Time-Correlated Single Photon Counting (TCSPC)

Microscopy capabilities

  • Single- and Multi-Photon Laser Scanning Confocal time-resolved fluorescence imaging
  • Structured Illumination Microscopy (SIM)
  • Scanning Near Field Optical Microscopy (SNOM)
  • Stimulated Emission Depletion Microscopy (STED)
  • Time Resolved Total Internal Reflection Fluorescence Microscopy (TIRFM) – Evanescent Wave Induced Fluorescence for FLIM and SLIM
  • Single Molecule Fluorescence Correlation Spectroscopy

Time-Resolved Fluorescence Spectroscopy and Anisotropy (Bulk)

Equipment

Femtosecond (Coherent Amplified System)

  • A mode-locked titanium sapphire laser (Mira-Seed: 20 fs, 76 MHz, 0.5 W)
  • Biophotonics Solutions MIIPS 640P pulse shaper
  • A chirped-pulse regenerative-amplifier (RegA9050: <50 fs, 10-250 kHz, 0.1-2.5 W)
  • Two optical parametric amplifiers (OPA9050: ~50 fs, [>nJ] 400 nm, [~0.15 nJ] 480-700 nm, [~20 pJ] 900-2300 nm)

Femtosecond (High-Repetition)

  • A cavity-dumped mode-locked titanium sapphire laser (Mira 900/PulseSwitch: ~120 fs, 76 MHz and 54 MHz to 200 Hz, 710-900 nm, ~40 nJ)
  • Doubler and Tripler (240-300 nm, 350-450 nm)
  • Optical Parametric Oscillator (APE: intracavity doubled)

Femtosecond (High-Repetition)

  • KML Ti:sapp. oscillator (15 fs @ 80 MHz)

Picosecond

  • Fianium SC450-PP-HE super-continuum laser
  • NKT super-continuum laser/Varia
  • Time-Bandwidth Products Cheetah X, frequency doubled picosecond Nd:Vanadate laser
  • Two cavity-dumped dye lasers (Spectra Physics)

Nanosecond

  • Q-Switched Nd:YAG (Continuum NY-61)
  • Optical parametric oscillator (Casix)
  • NKT SuperK COMPACT supercontinuum laser

Microscopes

  • Laser Scanning Confocal (Olympus IX71/FV300)
  • TIRF (Nikon TE2000)
  • Structured Illumination Microscopy
  • Scanning near field optical microscopy (SNOM) (Nanonics)
  • STED microscopy
  • Single Molecule
  • EMCCD (Roper)
  • sCMOS (Andor)

Time-resolved microscopy techniques

  • TCSPC (Becker and Hickl SPC830 & SPC150, EI TCC900 cards)
  • Gated photon counting (Nikon LIMO)
  • High repetition rate intensified CCD camera (La Vision PicoStarHR)

Misc

  • Liquid nitrogen/helium cryostats for low temperature measurements using any of the above techniques
  • Cary UV-Vis and Fluorescence spectrometers
  • Reflectance spectrometer
  • Quartz Crystal Microbalance